A total of 36,754,464 bp of sequence data was obtained from sample V5 that included 94,728 high quality 454 sequence reads, with a mean length of 388 bp. For the V6 sample, a total of 1,170,900 bp of sequence data was obtained that included 5,204 high quality reads, with a mean length of 225 bp. The lower quantity of sequence data for V6 might have resulted from lower nucleic acid concentrations (as we repored previously, [14], [15]), as well as higher degrees of degradation. Overall, approximately 15% of the sequences were unique, while the remaining 85% were additional copies from the unique set of sequences. A total of 3,369 unique sequences were derived from V5, of which 1,543 could be taxonomically classified (Figure 1; Tables S1, S2, S3, S4, S5, S6), and 138 unique assembled sequences were derived from V6, of which 80 could be taxonomically classified (Figure 1; Tables S7, S8, S9, S10). Approximately 94% of the unique sequences in V5 and 83% in V6 were from Bacteria. Only two unique Archaea sequences were found (both in V5), and they were most similar to methanotrophs from cold deep-ocean sediments. The remainder were Eukarya (4% in V5 and 17% in V6), including more than 150 unique sequences from multicellular organisms, most of which were Fungi. In general, the taxa were similar to organisms specific to lakes, brackish water, marine environments, soil, lake sediments, deep-sea sediments, deep-sea thermal vents, animals and plants (Figure 2). Sequences from autotrophs and heterotrophs were present. plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0067221
Posted on: Mon, 08 Jul 2013 03:39:27 +0000
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