Cell extract-containing medium for the culture of intracellular fastidious bacteria. ncbi.nlm.nih.gov/pubmed/23740722 J Clin Microbiol. 2013 Jun 5. [Epub ahead of print] Singh S, Kowalczewska M, Edouard S, Eldin C, Perreal C, Weber P, Azza S, Raoult D. Source Aix Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, 13005 Marseille, France. Abstract The culture of fastidious microorganisms is a critical step in infectious disease studies. As a proof-of-concept experiment, we evaluated an empirical medium containing eukaryotic cell extracts for its ability to support the growth of Coxiella burnetii. Here, we demonstrate the exponential growth of several bacterial strains, including the C. burnetii Nine Mile phase I and phase II strains and C. burnetii isolates from humans and animals. Low oxygen tension and the presence of small hydrophilic molecules and short peptides were critical for facilitating growth. Moreover, bacterial antigenicity was conserved, revealing the potential for this culture medium to be used in diagnostic tests and in the elaboration of vaccines for C. burnetii. We were also able to grow the majority of previously tested intracellular and fastidious bacteria, including Tropheryma whipplei, Mycobacterium bovis, Leptospira sp., Borrelia sp. and most putative bioterrorism agents. However, we were unable to culture Rickettsia africae and Legionella sp. in this medium. The versatility of this medium should encourage its use as a replacement for the cell-based culture systems currently being used for growing several facultative and putative intracellular bacteria.
Posted on: Fri, 07 Jun 2013 13:10:47 +0000
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