Exploiting Redundancy Same protein, same sets of amino acids but - TopicsExpress

Exploiting Redundancy Same protein, same sets of amino acids but coded by different codons impact the expression. How? Read the summary below: The genetic code is redundant—multiple codons can code for the same amino acid. So-called synonymous codon changes within genes can nonetheless have substantial affects on protein expression, which have been attributed to changes in the structure of 5′ messenger RNAs, among other factors. Goodman et al. built and measured the expression of a synthetic library of 14,000 variant N-terminal sequences of 137 Escherichia coli genes to show that, unexpectedly, rare codons had a bigger effect on increasing protein expression than more common codons. Increased RNA structure downstream of translation initiation appeared to represent the major determinant of expression differences owing to codon usage. Courtesy: Science

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