Three nucleotide databases, WGS, NCBI Genome and nr, with four - TopicsExpress

Three nucleotide databases, WGS, NCBI Genome and nr, with four different BLAST algorithms, Megablast, Discontiguous Megablast, Blastn, and TBLASTX, were applied for protocol validation. Each sequencing reactions from both positive controls were assembled into contig using DNA Baser Version 3.5.3(Heracle BioSoft S.R.L., România, Pitesti, jud Arges, str Banat, 110406) in order to determine whether there were any errors in the PCR Amplification/Direct DNA sequencing protocol. Finally, when the validated protocol was archived then the samples were performed downstream bioinformatic analyzes.

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