ELISA Kits ordered. ;) Dionex* Glyphosate Herbicide Analysis Kit Thermo Scientific* Dionex Glyphosate Herbicide Analysis Kit can be used for EPA Method 547 and AOAC Method 991.08. ELISA Kit for Agricultural Pollutants Glyphosate ELISA Kit (Magnetic Particle Format) ◊ The antibody is specific for Glyphosate an d does not cross-react with other non-related agricultural compounds. ◊ The assay sensitivity is 0.05 ppb. The assay range is between 0.075 ppb and 4.0 ppb. This supersensitive assay allows the determination of Glyphosate in a range of environmental samples (water, soil, sediment, fish pl asma, etc.). ◊ No time-consuming sample extraction. ◊ Total time for measurement is less than 90 minutes. ◊ The kit (120 Tests), a magnetic particle form at with ready to use re agents, enables faster assay kinetics, super sensitivity, and the simultaneous measurement of multiple samples at a reasonable cost. Chemical Structure Glyphosate is a broad spectrum non-selective herbicide which kills all plants, including grasses, broad leaf and woody plants. Because of it’s broad spectrum in action, it is used to control a great variety of annual, biennial, and pe rennial grasses, sedges, broad leafed weeds, and woody brush. It is also used in fruit orchards, vineyards, conifer plantation and many plantation crops (e.g. coffee, tea, bananas); in pre-crop, and post-weed emer gence in a wide range of crops (including soybeans, cereals, vegetables and cotton); on non-crop areas (e.g. road shoulders and rights of way); in cereal stubble; forestry; gardening and horticulture. Using traditional analytical methods, Glyphosate is extremely difficult to me asure in environmental samples. Only a few laboratories have the sophisticated equipment an d techniques necessary. This ELISA test kit offers a quick, easy, and cost- effective method to detect Glyphosate in environment samples at the ppt levels. Glyphosate Standard Curve Samples containing Glyphosate within the range (0.075-4.0 ppb) can be tested in the assay after filtration and quick derivatization. Concentration (ppb) 0.1 1 10 B/Bo (%) 10 20 30 40 50 60 70 80 90 Basic Test procedure • Derivatize sample for 10 minutes • Add 300 uL of sample, and 500 uL of ant ibody coupled magnetic particles. Vortex. • Incubate for 30 minutes. • Add 250 uL of conjugate. Vo rtex and incubate for 30 minutes. • Separate using the magnetic separator, decant and wash. • Add 500 uL of color solution. • Incubate 20 minutes. • Stop the reaction and read color at 450 nm. Quantitate results. Cross-reactivity Pattern Cross-reactivity of the Abra xis Glyphosate ELISA expressed as the least detectable dose (LDD) which is estimated at 90% B/Bo and at the concentration required to displace 50% (50% B/Bo). LDD 50% B/Bo Compound (ppb) (ppb) ________________________________________________________ Glyphosate 0.10 2.40 Glyphosine 50 3,000 Glufosinate 2,000 70,000 AMPA 35,000 >1,000,000 Glycine >10,000 >1,000,000 ________________________________________________________ The following compounds demonstrated no reactivi ty in the Glyphosate Assay when tested at concentrations up to 1,000 ppb: aldicarb, aldicarb sulfoxide, aldicarb sulfone, acetochlor, alachlor, atrazine, amet ryn benomyl, butachlor, butylate , captan, carbaryl, carbendazim, carbofuran, 2,4-D, 1,3-dichlo ropropene, dinoseb, MCPA, me tribuzin, metolachlor, PCP, picloram, propazine, sima zine, terbufos, thiabendazole, thiophanate-methyl. Kit Format Glyphosate ELISA Kit (Magnetic Particle format, 120T) PN 500081 Manufactured by Abraxis LLC 54 Steamwhistle Drive Warminster, PA 18974 Phone: (215) 357-3911 FAX: (215) 357-5232 Email: info@abraxiskits WEB: abraxiskits
Posted on: Tue, 30 Jul 2013 14:53:19 +0000
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