J Sci Food Agric. 2013 Aug 8. doi: 10.1002/jsfa.6340. [Epub ahead - TopicsExpress

J Sci Food Agric. 2013 Aug 8. doi: 10.1002/jsfa.6340. [Epub ahead of print] An in vitro study reveals nutraceutical properties of Ananas comosus (L.) Merr. var. Mauritius residue beneficial to diabetes. Riya MP, Antu KA, Vinu T, Chandrakanth KC, Anilkumar KS, Raghu KG. SourceAgroprocessing and Natural Products Division, Council of Scientific and Industrial Research -National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram, - 695019, Kerala, India. Abstract BACKGROUND: Rapid urbanization and nutritional transition is fuelling the increased global incidence of type 2 diabetes. Pineapple fruit residue was explored for its nutraceutical properties as an alternative or adjunct to currently available treatment regime. Ethyl acetate (PAE) and methanolic extracts (PAM) of pineapple fruit residue were evaluated for antidiabetic activity in cell free and cell based systems. Specifically, we assessed: (1) antioxidant potential (2) antiglycation potential (3) carbohydrate digestive enzyme inhibition and (4) lipid accumulation and glycerol-3-phosphate dehydrogenase (GPDH) activity in differentiating 3T3-L1 cells. RESULTS: The active components in the ethyl acetate and methanolic extracts were identified as sinapic acid, daucosterol, 2-methylpropanoate, 2, 5-dimethyl-4-hydroxy-3(2H)-furanone, methyl 2-methylbutanoate and triterpenoid ergosterol using DART/HRMS and ESI/HRMS. Micronutrient analysis revealed the presence of magnesium, potassium & calcium. Adipogenic potential, antiglycation property of the PAE and DNA damage protection capacity of PAM are promising. CONCLUSION: Results from this study clearly indicate that pineapple fruit residue could be utilized as a nutraceutical against diabetes and related complications. This article is protected by copyright. All rights reserved. KEYWORDS: Adipogenesis, Alpha glucosidase, Ananas comosus (L.) Merr. var. Mauritius, Antiglycation, Antioxidant, Bromeliaceae, Pineapple fruit residue

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