NEW PRODUCT OF THE WEEK - New England Biolabs: M0371S Shrimp - TopicsExpress

NEW PRODUCT OF THE WEEK - New England Biolabs: M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units So it begs the question which alkaline phosphatase works best?? rSAP, CIP or Antarctic Phosphatase? Well here is your answer folks! Shrimp Alkaline Phosphatase or rSAP (NEB #M0371) is a superior choice, because it combines the advantages of both Alkaline Phosphatase, Calf Intestinal (CIP) (NEB #M0290) and Antarctic Phosphatase (NEB #M0289). rSAP has a very high specific activity, comparable to CIP, with the advantage of being heat inactivatable, unlike CIP. rSAP is inactivated, as is Antarctic Phosphatase, but rSAP is preferred over Antarctic Phosphatase, because it does not require the user to add zinc or other co-factors. As a result, rSAP can be added directly to restriction enzyme digests. Additionally, after heat-inactivation of rSAP, it is not necessary to purify vector DNA prior to the ligation reaction. To order visit genesearch.au

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