Review for 3722 Sections 4 & 8 Practice questions. Try to answer these without your notes or book: What is the BSL of this lab? What is the procedure for cleaning up a spill with broken glass? What is the resolving power of a fluorescent microscope? What is the total magnification when using the 10x objective lens? What tool is used for the spread plate? What are two examples of a basic stain? What is the primary stain of the Gram stain? The counterstain? What is the pH indicator in the snyder medium? Which selective plates are selective for Gram-negatives? What is the carbohydrate in the MAC plate? List all reagents added to the nitrate test after incubation. What enzyme are you testing for with the milk agar plate? Describe the appearance of a positive catalase test. Which test can be used to identify a Group B Streptococcus? Which carbohydrates are present in the TSI slant? What are their respective concentrations? Why would a bacterium produce a spore? How many colonies are on a countable plate? What is the mechanism of action of polymixin B? What class does pilobolus belong to? Which parasitic helminth causes cysticercosis? Anything you have seen during the semester could be on the exam. I will primarily be testing your knowledge of tests (ingredients, inoculation, incubation, reagents, results, etc), procedures, and the eukaryotic microbes (fungi, protozoans, helminths). There will be at least eight stations that involve reading a biochemical test, and at least five that involve identifying and describing a eukaryotic microbe. For the biochemical tests, make sure you know how to read the results, not just positive or negative, but what it means (a certain enzyme produced or reaction that has taken place). Knowing the substrates and pH indicators will help greatly here. Also, know all of the procedures you did in class, including how to inoculate each medium, how to do things aseptically, what to do in case of a spill, and how to do the single and double unknowns. Finally, you should be able to identify any of the eukaryotic microbes on the slides — any that you see on the exam will look similar to the ones you saw in class, and you should also be able to tell me something about it once you have identified it. Dont forget to show up in the correct clothing, just like any other day.
Posted on: Mon, 28 Apr 2014 03:46:30 +0000
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