TORC15 CONFERENCE NEWS --->> Speaker Announcement Key words: Paphiopedilum parishii; endangered species; conservation; tissue culture; shoot multiplication Bo Long﹡1, Wenjun Su2 1 School of Life Sciences, Yunnan University, Kunming, China 2 Biology Center, The first People’s Hospital of Kunming, China Efficient in vitro propagation method with increased shoot organogenesis of endangered Paphiopedilum parishii Abstract: Paphiopedilum spp. (Orchidaceae) of the subfamily Cypripedioideae (Lady’s slipper orchids), are sold as attractive plants on the horticulture market. The purpose of this study was to provide a pragmatic propagation method for P. parishii (Rchb. f.) Stein. P. parishii grows in broad-leaved forests of Southern Yunnan. It is a critically endangered species (Vol.1 of China’s Red List Species & CITES Appendix 1) due to habitat destruction, over-collection of the native plant, and subsequent large-scale illegal trade. The frequency of regular seed germination by P. parishii is very low. To grow a new plant in nature, 4-5 years are needed. However, native plant supply cannot meet the horticultural market demands. Therefore, in order to conserve this specie and avoid further destruction of remaining native plants, it is necessary to propagate them rather in horticulture. Different combinations of conditions of plant growth regulators (PGRs) on seed germination and shoot multiplication were investigated. We observed that seed germination was higher under such controlled conditions than in the wild. The explants showed a double increase in the frequency of shoot organogenesis. The use of plantlets, derived by this in vitro seed germination method for shoot multiplication shorten therefore the reproduction cycle and offer an attractive horticulture application. In consequence, our results offer new means for the conservation of P. parishii. This research was funded by a grant from the National Natural Science Foundation of China (31100249). Map: Courtesy Royal Botanical Gardens Kew
Posted on: Sun, 21 Dec 2014 08:37:45 +0000
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