Vaccine Production With - Human Diploid Cells (aborted fetal cell tissue) vacfacts.info/vaccine-production-with---human-diploid-cells-aborted-fetal-cell---tissue.html MRC-5, WI-38, DNA, MRC-5 Cellular Protein, Human Serum Albumin (all different ingredients)- All of these derive from either human tissue or human blood. Human fetal lung strain MRC-5- ncbi.nlm.nih.gov/pmc/articles/PMC274969/ MRC-5, 14 weeks gestation male caucasian fetus atcc.org/products/all/CCL-171.aspx WI-38, 3 months gestation female caucasian fetus atcc.org/products/all/CCL-75.aspx Aborted fetal cell line information: cogforlife.org/fetalvaccinetruth.htm (Sorry, some of these links are broken, and was taken from a recent post found on FB. Will attempt to get the full url links, later). To date, there are two human diploid cell lines which were originally prepared from tissues of aborted foetuses and are used for the preparation of vaccines based on live attenuated virus: the first one is the WI-38 line with human diploid lung fibroblasts, coming from a female foetus that was aborted because the family felt they had too many children. The second human cell line is MRC-5 (Medical Research Council 5) (human, lung, embryonic) with human lung fibroblasts coming from a 14 week male foetus aborted for psychiatric reasons from a 27 year old woman in the UK. immunize.org/concerns/vaticandocument.htm Fetal Bovine Serum/Calf Serum Protein- a product that is cruelly derived from the fetuses of cows found pregnant at slaughter. Serum is blood without any cells, platelets or clotting factors and fetal calf serum especially, is considered to be a rich source of nutrients. It is harvested from bovine fetuses taken from pregnant cows during slaughter. FBS is commonly harvested by means of a cardiac puncture without any form of anaesthesia. Fetuses are probably exposed to pain and/or discomfort, so the current practice of fetal blood harvesting is inhumane. ncbi.nlm.nih.gov/pubmed/11971757 Cow components are often used simply because cows are very large animals, commonly used for food, and thus much material is available. Animal-derived products used in vaccine manufacture can include amino acids, glycerol, detergents, gelatin, enzymes and blood. Cow milk is a source of amino acids, and sugars such as galactose. Cow tallow derivatives used in vaccine manufacture include glycerol. Gelatin and some amino acids come from cow bones. Cow skeletal muscle is used to prepare broths used in certain complex media. fda.gov/.../QuestionsaboutVaccines/ucm143521.htm Some cows brought to slaughter are pregnant. After being slaughtered, their fetus - still alive - is extracted from the uterus by incision. A needle is then plunged deep into the heart, whose beats can draw blood without effort. This intracardiac puncture, deemed extremely painful and causes a slow death by asphyxiation. occupyforanimals.org/fetal-bovine-serum-or... Embryonic Guinea Pig Cell Cultures- The virus was initially obtained from a child with natural varicella, introduced into human embryonic lung cell cultures, adapted to and propagated in embryonic guinea pig cell cultures, and finally propagated in human diploid cell cultures. nvic.org/vacci.../chickenpox/chickenpoxfacts.aspx Immunization of inbred guinea pigs with Varicella-Zoster virus ncbi.nlm.nih.gov/pmc/articles/PMC271940/ Organism: Cavia porcellus, guinea pig / Tissue: fetus atcc.org/products/all/CRL-1405.aspx The use of fetal bovine serum: ethical or scientific problem? ncbi.nlm.nih.gov/m/pubmed/11971757/ vaccines.procon.org/view.resource.php... Aborted fetal cells? All you have to do is read the vaccine insert. How about this from Mercks own website? merck/.../pi_circulars/m/mmr_ii/mmr_ii_pi.pdf Under the Description in the opening paragraph, note the reference to Footnote 1,2. Scroll down to page 9 and read the first footnote under References Direct quote from the CDC vaccine ingredient list This table includes not only vaccine ingredients (e.g., adjuvants and preservatives), but also substances used during the manufacturing process, including vaccine-production media, that are removed from the final product and present only in trace quantities. Not MAY be present but ARE present. The moral dilemma of vaccines being cultured in the cells is enough for me, but according to the CDC they ARE present in the final product. In some cases the cell lines (aborted fetal cell lines) that are used might be tumorigenic, that is, they form tumors when injected into rodents. Some of these tumor-forming cell lines may contain cancer-causing viruses that are not actively reproducing. Such viruses are hard to detect using standard methods. These latent, or quiet, viruses pose a potential threat, since they might become active under vaccine manufacturing conditions.” fda.gov/.../biologicsresearchareas/ucm127327.htm Xenotropic murine leukemia virus-related virus (XMRV) is a recently discovered human retrovirus that has been found in both chronic fatigue syndrome & prostate cancer patients. There is a potential safety concern regarding XMRV in cell substrates used in vaccines... fda.gov/.../biologicsresearchareas/ucm127327.htm The use of tumorigenic and tumor-derived cells is a major safety concerndue to the potential presence of viruses such as retroviruses and oncogenic (CANCER) DNA viruses that could be associated with tumorigencity, Therefore, detection of persistent, latent DNA viruses, and endogenous retroviruses in vaccine cell substrates is important for vaccine safety, particularly in the development of live viral vaccines, where there are no or minimal virus inactivation and removal steps during vaccine manufacturing. fda.gov/.../biologicsresearchareas/ucm127327.htm Regulatory evaluation of transformed cell substrates could be improved by a better understanding of the processes involved in cell transformation. Thus, our laboratory is attempting to understand mammalian cell transformation, how transformed cells develop the ability to form tumors, and how these tumors actually develop. Our laboratory is also developing ways to evaluate the possible risks that might be associated with the genetic material (DNA) from tumor-forming cell substrates. DNA from these substrates poses two risks: 1) the possibility of transferring cancer-causing activity to vaccines, and 2) the possibility of transferring infectious microorganisms to vaccines. To extend our work to include all aspects of the risks associated with tumor-forming cell substrates, we are developing ways to 1) determine whether DNA from such cells can be a source of cancer-causing activity and infectious microorganisms; and 2) evaluate the impact of addition of enzymes during manufacturing that would cause DNA degradation (breakdown) on the relative risks associated with these processes. fda.gov/.../BiologicsResearchAreas/ucm127304.htm
Posted on: Tue, 06 Jan 2015 23:43:38 +0000
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