glorybioscience/ Porcine Plasminogen Activator Inhibitor 1 (PAI-1) ELISA Kit FOR RESEARCH USE ONLY. Not for clinical diagnosis use CATALOG #:50126 INTRODUCTION For the quantitative determination of porcine PAI-1 concentrations. Detection of species: Porcine Detection medium: serum, plasma, tissue homogenates, cell culture supernates. PRINCIPLE OF TEST The kit is for the quantitative level of PAI-1 in the sample, adopt purified porcine PAI-1 to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to PAI-1, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody - antigen - enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of PAI-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. DETECTION RANGE Detection range of the kit is: 0.5ng/mL -20ng/mL
Posted on: Tue, 16 Dec 2014 07:06:04 +0000
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