glorybioscience/ Rat Catalase (CAT) ELISA Kit FOR RESEARCH USE ONLY. Not for clinical diagnosis use CATALOG #:31770 INTRODUCTION For the quantitative determination of rat CAT concentrations. Detection of species: Rat Detection medium: serum, plasma, tissue homogenates, cell culture supernates. PRINCIPLE OF TEST The kit is for the quantitative level of CAT in the sample, adopt purified rat CAT to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to CAT, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody - antigen - enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of CAT in the samples is then determined by comparing the O.D. of the samples to the standard curve. DETECTION RANGE Detection range of the kit is: 0.1U/L - 4U/L
Posted on: Thu, 11 Dec 2014 07:03:41 +0000