Determination of Triterpenes in Centella asiatica (Gotu Kola) by HPLC-CAD Mark Roman Tampa Bay Analytical Research, Inc., Largo, FL 33777 Introduction Centella asiatica (commonly called gotu kola) is a small herbaceous annual plant native to India, Sri Lanka, Northern Australia, other parts of Asia, and the Western Pacific. It is used as a medicinal herb in Ayurvedic medicine and in traditional Chinese medicine to treat a wide variety of conditions, such as improving memory, blood flow, as a wound-healing agent, and as a topical application for skin conditions such as ulcers, wounds, and eczema. The chemical compounds of interest in gotu kola are usually considered to be the ursane- and oleanane-type triterpenes and the triterpene glycosides including asiaticoside, madecassoside (asiaticoside A), asiaticoside B, madecassic acid, asiatic acid, and terminolic acid . Most methods for the analysis of the total triterpenes use gradient elution reversed-phase high-performance liquid chromatography (RP-HPLC) with low-wavelength UV detection (203–205 nm) because the triterpenes do not strongly absorb above 205 nm. This often results in strongly sloping baselines, and minor components that have stronger UV chromophores than the triterpenes can cause interferences that affect quantitation. The Thermo Scientific Dionex Corona™ CAD™ Charged Aerosol Detector offers an alternative to low-wavelength UV detection to improve the baseline and reduce interferences. Here, chromatograms generated using UV and Corona CAD detection are compared, and the assay of triterpene content in a Centella asiatica extract sample using the Corona CAD detector is shown.
Posted on: Tue, 10 Sep 2013 11:18:50 +0000
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