Maytenus Robusta Reissek is a primary healthcare medicine which has a long ethnomedical in Brazil as it contains compounds that treat gastric ulcers which have been clinically tested against omeprazole (gastrogard) cimetidine and ranitidine. The good thing about using a plant rather than a drug to help EGUS is that plants (and this pant especially) work synergistically with the body rather than destroying parts of it. This shot gun effect is better as the end result is a stronger gastrointestinal tract. This plant is grown as a medicinal crop and our vision is to replace it with a UK plant to develop a sustainable supply line and to support the UK economy depletion of the rain forests. Heather contains the compound we want and below is the first trial (equine) to find the clinical evidence to support further research, this will be followed by a trial to compare the effects of the Brazilian plant and the UK plant. Description of the trial-Maytenus anti-inflammatory and anti-oxidant screening test: Intestinal cell culture The aim of the study is to determine the anti-inflammatory and anti-oxidant properties of Maytenus. This will be achieved using an in vitro intestinal cell culture model of inflammation. Briefly, the methodology already established is outlined: The intestinal cell line has already been developed, optimised and established by Manuela Natoli of IBERS. The cells will be cultured until confluent and thereafter the culture medium is removed and cells seeded into wells of a 96-well culture plate. Fresh medium will be added to each well containing one of the following treatments: fresh medium (control) or fresh medium containing a treatment to stimulate inflammation in the cells (for example, E.coli-derived lipopolysaccharide) and +/- Mayotenus using a high or low dose. Treatments will be tested in triplicate and the experiment conducted on three separate occasions. To confirm that an inflammatory response was achieved, and to determine whether Maytenus abrogated the response (anti-inflammatory effect), samples of medium are collected 24 and 48 hours after challenge from each well and analysed for inflammatory markers. The marker of inflammation that will be measured from spent culture medium will be prostaglandin F2α, using radioimmunoassay, which is already established in our laboratory. To test the anti-oxidant effect, nitric oxide and glutathione will be measured from spent cell culture medium by commercially available ELISA kits We have a list of plants for other major equine disease states that we plant to investigate so if you would like to sign up to monthly updates which will include information on foraging, plants from other countries under investigation and dosage please either PM your email or attach it to this post.
Posted on: Fri, 08 Aug 2014 06:16:16 +0000
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