Scientific Peer Reveiwed Evidence that Biomedical Intervention using GF/CF diet is effective in a sub group of ASD children :) Proof that in specific cases Gluten and Casien actually tiggered ASD. Long awaited report and much appreciated :) ANTIBODIES AGAINST FOOD ANTIGENS IN PATIENTS WITH AUTISTIC SPECTRUM DISORDERS (ASDs) Laura de Magistris, Annarita Picardi, Dario Siniscalco, Maria Pia Riccio, Anna Sapone, Rita Cariello, Salvatore Abbadessa, Nicola Medici, Karen M. Lammers, Chiara Schiraldi, Patrizia Iardino, Rosa Marotta, Carlo Tolone, Alessio Fasano, Antonio Pascotto, Carmela Bravaccio Laura de Magistris, PhD, Anna Sapone, MD, PhD Rita Cariello, PhD, CIRANAD, Second University of Naples, Italy Annarita Picardi, PhD, “Alimenti e Salute” PhD School, Dept. of Experimental Medicine, Second University of Naples, Italy Dario Siniscalco, ChemD, PhD, Dept. of Experimental Medicine, Second University of Naples and Centre for Autism - La Forza del Silenzio, Caserta, Italy Maria Pia Riccio, MD, Antonio Pascotto, MD, Prof, Dept. of Psychiatry, Neuropsychiatry and Dermatovenereology, Second University of Naples, Italy Salvatore Abbadessa, PhD, Nicola Medici, MD, Prof, Dept. of General Pathology, Second University of Naples, Italy Karen M Lammers, PhD, Alessio Fasano, MD, Prof, Center for Celiac Research and Mucosal Immunology and Biology Research Center , Massachusetts General Hospital, Charlestown, MA, USA Chiara Schiraldi, PhD, Dept. of Experimental Medicine, Second University of Naples, Italy Patrizia Iardino, PhD, UOC Clinical and Molecular Pathology, Second University of Naples, Italy Rosa Marotta, MD, Dept. of Psychiatry, University of Catanzaro “Magna Graecia”, Catanzaro, Italy Carlo Tolone, MD, Prof, Dept of Pediatrics, Second University of Naples, Italy Carmela Bravaccio, MD, Prof, Dept. of Pediatrics, University of Naples “Federico II”, Italy Running title: Food antigens antibodies in autism Corresponding author: Laura de Magistris, Prof, PhD CIRANAD, Second University of Naples, Italy, via Pansini, 5 – Building 3 – Naples, 80138 Italy. email: [email protected] Telephone: +39 (0)81-5666740/5665116 Fax: +39 (0)81-5665112/5666732 2 ABSTRACT Purpose: Many individuals who suffer from autism spectrum disorders (ASDs) show an impaired intestinal barrier function. The increased intestinal permeability of a large percentage of non-celiac autistic individuals appears to be normalized by implementation of gluten- and casein-free diet (GF/CF). We hypothesize that immune system of some autistic patients is abnormally triggered by gluten/casein assumption. We sought to describe the prevalence of antibodies to a series of food antigens, including gliadin and milk proteins, in autistic children with paired/impaired intestinal permeability and under dietary regimen either regular or restricted (GF/CF). Methods: 162 ASDs and 44 healthy children, were investigated for: Intestinal permeability, assessed with the lactulose/mannitol test; Tissue-transglutaminase (tTG), Anti-endomysium antidodies (EMA)-IgA and Total mucosal (IgA) to exclude Celiac Disease; HLA-DQ2/-DQ8 haplotypes; Total systemic antibodies (IgA, IgG, IgE); Specific systemic antibodies: α-gliadin (AGA-IgA and IgG), deamidated-α-gliadin-peptide (DGP-IgA and IgG), total specific gliadin IgG (all fractions: α,β,γ,ω), β-Lactoglobulin IgG, α-Lactoalbumin IgG, Casein IgG, and Milk IgE, Casein IgE, Gluten IgE, β-Lactoglobulin IgE, α-Lactoalbumin IgE. Results: AGA-IgG (40.4 ± 3.1 U/ml) and DPG-IgG (4.87 ± 0.6 U/ml) titers are higher in ASDs compared to controls (22.4 ± 3.4; p
Posted on: Tue, 02 Jul 2013 09:38:49 +0000
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