The development of a chronic mollusc toxicity test is currently a work item on the agenda of the OECD. We report the improvement and standardization of a toxicity bioassay using eggs of the great pond water snail Lymnaea stagnalis L., which have a biological indicator of water pollutants. We optimized both isolation and synchronization methods to reduce variability, and identified sensitive developmental stages and parameters to assess the putative toxicological effects (i.e. heartbeat, period of development, hatching success, mortality and movement). Our work is an invaluable advancement to develop a reliable, fast and inexpensive environmental embryo toxicity test for molluscan species.
Posted on: Mon, 17 Nov 2014 13:12:54 +0000
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