glorybioscience Human Thyroid Stimulating Hormone (TSH) ELISA Kit FOR RESEARCH USE ONLY. Not for clinical diagnosis use CATALOG #:10717 INTRODUCTION For the quantitative determination of human TSH concentrations. Detection of species: Human Detection medium: serum, plasma, tissue homogenates, cell culture supernates. PRINCIPLE OF TEST The kit is for the quantitative level of TSH in the sample, adopt purified human TSH to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to TSH, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody - antigen - enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of TSH in the samples is then determined by comparing the O.D. of the samples to the standard curve. DETECTION RANGE Detection range of the kit is: 0.1 mIU/L -6 mIU/L
Posted on: Thu, 15 Jan 2015 05:29:38 +0000
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