RESULTS(2) Direct DNA sequencing and Bioinformatics Analysis Figure 5 LAMP target region amplified by F2-B2 PCR with F2-B2-104 primers. LAMP Product was used as DNA template for F2-B2 PCR to amplify. LAMP target. Then it was subjected to direct DNA sequencing. Table 2 BLAST Hits of reference C. krusei DMST 15317. The sequence from LAMP target were analyzed for sequence similarity by BLAST. BLAST Hits were determined with E-value. Figure 6 Mapped-base alignment against known C. krusei SSU rDNA reference sequence. Nucleotide sequences from C. krusei LAMP target were assembled to known C. krusei SSU rDNA reference sequence in order that the sequence similarity can be performed. Table 3 BLAST Hits of selected clinical strains. The sequence from LAMP target and PCR products were analyzed for sequence similarity by BLAST. BLAST Hits were determined with E-value.
Posted on: Wed, 24 Jul 2013 04:28:58 +0000
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