Dear Kary~as you mentioned~there is another independent measure - TopicsExpress



          

Dear Kary~as you mentioned~there is another independent measure not related to DNA~but its still BLOOD related- using an EM to photograph the blood to detect the virus named “HI.” How come I can have a viral load of 9 million copies/mL using your PCR method, and not one virus show in the blood? What sequence are they using? And how can YOU prove this? EM is the ultimate method ~ but we need to disprove the sequence of “HIV.” Trisha Harding Morrison, Date: Sat, May 11, 2013 5:55 pm Dear Tommy (and Trisha), A number of diseases are detected by the fact that using two sequences known to be contained in the DNA/RNA of some organism and running a PCR reaction, containing that DNA, a fragment of a known size predictable from the sequence of that organism will be produced. Further evidence (and much more credible) for the presence of that particular organism can be obtained by sequencing the fragment so obtained. An intermediate level of confidence can be obtained by so-called hybridization of the obtained fragment with a known sequence standard. Most diseases can be diagnosed with some independent measure not related to DNA, but over the years DNA evidence has become a well-trusted method, and the mere presence of HIV DNA sequences in your blood is sufficient cause for most physicians to pronounce that you have AIDS and treat you for it. (You can have your prostate gland surgically removed based on higher than expected levels of prostate hormone, but I wouldn’t recommend it without collaborative evidence of rampant prostate cancer.) The issue of assigning meaning to the evidence of HIV sequences in people is in my opinion the real outstanding issue, and my opinion is that there is insufficient evidence to conclude that such sequences are dangerous enough to the person (if at all) to justify a treatment that may very well be. I have made myself clear on this issue for years. If I were to discover something which would make me think otherwise, I would feel honor-bound to make that very public. Sadly anybody who can read and really wants to know about the issue of AIDS can read about it forever and still not know much about it as it is a highly contested issue and the financial consequences are immense. There are a large number of such issues in the world. Welcome to Earth. PCR detects a very small segment of the nucleic acid which is part of the virus itself. The specific fragment detected is determined by the somewhat arbitrary choice of DNA primers used which become the ends of the amplified fragment (not virus isolation). They have to be in the sequence for it to be amplified in the first place, but they can be rather a small part of the total sequence. (Two to three hundred nucleotides is usually chosen out of several thousand in the total retrovirus). When incorporated in a cell the virus exists as DNA, when it is released from the cell in its infectious form it is RNA; which can be converted easily into DNA in the cell or in vitro for purposes of amplification. There are many sequence variations among the sequences called HIV. Any one of them can get you classed as what they consider HIV positive. And due to the tiny amounts of nucleic acid detectable after many cycles of PCR amplification (after 30 cycles one copy will get you about a billion copies) the test is super sensitive. Antibodies are detected by a non-PCR test called an EIisa. Elisa testing is not so specific as PCR as there are many antibodies not specific to HIV which may cross-react in this test. If a sequence amplifies with primers designed to HIV, it is HIV by definition. In order to amplify and produce a fragment of approximately the correct size the original sequence must hybridize with the primers, and therefore it must be at least a very close match (at least at the primer sites) to what has been defined as HIV. (now called HIV-AIDS in case there are any skeptics). Kary Mullis, Date: Tue, May 7, 2013 4:44 pm blogs.naturalnews/wife-of-tommy-morrison-boxing-champion-dead-at-44-cites-numerous-illnesses-not-associated-with-hiv/
Posted on: Sun, 08 Sep 2013 15:12:27 +0000

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