PCR Tips: Touchdown!! oak.ctx.ly/r/1h5pf Touchdown PCR involves decreasing the annealing temp by 1°C every second cycle to a ‘touchdown’ annealing temp which is then used for the remaining cycles. Touchdown PCR increases specificity & reduces background amplification. By starting at a high annealing temp only your gene of interest is amplified, allowing the target product to accumulate. Decreasing the annealing temp through the remaining PCR cycles permits efficient amplification of the specific template.
Posted on: Sat, 02 Aug 2014 16:32:05 +0000
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